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How-To Guide

Reconstitution and injection procedures for research peptides.

Research Use Only. All products sold by Pretty Peptides Co. are intended strictly for laboratory research use only. They are not intended for human or veterinary consumption, diagnosis, treatment, or prevention of any disease. The procedures below describe standard laboratory reconstitution and subcutaneous administration technique for in-vitro research purposes only.

What you'll need

  • Lyophilized peptide vial
  • Bacteriostatic water (0.9% benzyl alcohol)
  • One reconstitution syringe (3mL recommended)
  • U-100 insulin syringes (100 unit / 1mL)
  • Alcohol prep wipes
  • Sharps disposal container

Reconstitution — adding BAC water

  1. 1

    Wash and prep

    Wash hands thoroughly. Lay out a clean surface with the peptide vial, BAC water, syringes, and alcohol wipes. Inspect each vial — the peptide should be a uniform powder or cake, with no discoloration.
  2. 2

    Sanitize the stoppers

    Remove the plastic flip-top from both the BAC water vial and the peptide vial. Wipe each rubber stopper with a fresh alcohol prep pad and let it air-dry for a few seconds.
  3. 3

    Draw BAC water

    Using your reconstitution syringe, pull back the plunger to draw in the desired volume of air. Insert the needle into the BAC water vial, push the air in, invert the vial, and slowly draw the planned amount of BAC water (commonly 1mL, 2mL, or 3mL depending on the dose math from the calculator).
  4. 4

    Inject slowly into the peptide vial

    Insert the needle into the peptide vial and angle the tip against the inside wall of the glass. Push the plunger down slowly so the BAC water runs down the glass wall onto the peptide — do not blast it directly onto the powder.
  5. 5

    Swirl, do not shake

    Gently swirl the vial in a circular motion until the powder fully dissolves. Never shake — peptides are fragile and shaking can denature them. If it doesn't dissolve in 30–60 seconds, set it aside for a couple minutes and swirl again.
  6. 6

    Label and store

    Write the reconstitution date on the vial label. Store reconstituted peptides in the refrigerator (2–8°C / 36–46°F). Most reconstituted research peptides are stable for 4–6 weeks refrigerated; check the COA for product-specific guidance.

Calculating your dose

The volume you draw depends on how much BAC water you added and the target dose. Use our calculator to get exact unit markings on a U-100 syringe.

Open the peptide reconstitution calculator →

Troubleshooting

  • Powder won't dissolve: swirl gently and wait — never shake. Verify the BAC water actually entered the vial.
  • Cloudy or discolored solution: do not use. Discard the vial.
  • Resistance or leakage at the site: withdraw, reassess site selection, and use a fresh syringe.
  • Small bruise at site: normal and harmless — rotate sites next time.