How-To Guide

What you'll need

• Lyophilized peptide vial
• Bacteriostatic water (0.9% benzyl alcohol)
• One reconstitution syringe (3mL recommended)
• U-100 insulin syringes (100 unit / 1mL)
• Alcohol prep wipes
• Sharps disposal container

Reconstitution — adding BAC water

1. 1

   Wash and prep

   Wash hands thoroughly. Lay out a clean surface with the peptide vial, BAC water, syringes, and alcohol wipes. Inspect each vial — the peptide should be a uniform powder or cake, with no discoloration.
2. 2

   Sanitize the stoppers

   Remove the plastic flip-top from both the BAC water vial and the peptide vial. Wipe each rubber stopper with a fresh alcohol prep pad and let it air-dry for a few seconds.
3. 3

   Draw BAC water

   Using your reconstitution syringe, pull back the plunger to draw in the desired volume of air. Insert the needle into the BAC water vial, push the air in, invert the vial, and slowly draw the planned amount of BAC water (commonly 1mL, 2mL, or 3mL depending on the dose math from the calculator).
4. 4

   Inject slowly into the peptide vial

   Insert the needle into the peptide vial and angle the tip against the inside wall of the glass. Push the plunger down slowly so the BAC water runs down the glass wall onto the peptide — do not blast it directly onto the powder.
5. 5

   Swirl, do not shake

   Gently swirl the vial in a circular motion until the powder fully dissolves. Never shake — peptides are fragile and shaking can denature them. If it doesn't dissolve in 30–60 seconds, set it aside for a couple minutes and swirl again.
6. 6

   Label and store

   Write the reconstitution date on the vial label. Store reconstituted peptides in the refrigerator (2–8°C / 36–46°F). Most reconstituted research peptides are stable for 4–6 weeks refrigerated; check the COA for product-specific guidance.

Calculating your dose

The volume you draw depends on how much BAC water you added and the target dose. Use our calculator to get exact unit markings on a U-100 syringe.

Open the peptide reconstitution calculator →

Subcutaneous administration procedure

The following describes standard laboratory subcutaneous administration technique for in-vitro research use only.

1. 1

   Select an administration site

   Identify a designated subcutaneous administration site on the research subject per your protocol. Rotate sites between administrations to minimize localized irritation and maintain consistency across data collection.
2. 2

   Clean the site

   Wipe the selected site with an alcohol prep pad in a circular motion outward. Allow it to fully air-dry before administration.
3. 3

   Sanitize the vial stopper

   Wipe the reconstituted peptide vial's rubber stopper with a fresh alcohol pad.
4. 4

   Draw the dose

   Pull air into the U-100 insulin syringe equal to the dose volume. Insert the needle into the upright vial, push the air in, invert the vial, and slowly draw to your target unit mark. Tap out air bubbles and push them back into the vial, then redraw to your exact mark.
5. 5

   Insert the needle

   Insert the needle into the prepared subcutaneous site at a 45° to 90° angle in a single smooth motion. U-100 insulin syringes have short needles designed to deposit volume into the subcutaneous layer.
6. 6

   Administer and withdraw

   Press the plunger steadily until the syringe is empty. Pause briefly, then withdraw the needle at the same angle of insertion. Apply light pressure at the site with a clean cotton ball or fresh alcohol pad.
7. 7

   Dispose safely

   Place the used syringe directly into a sharps container. Never recap, bend, or reuse needles. Return the peptide vial to refrigeration.

Troubleshooting

• Powder won't dissolve: swirl gently and wait — never shake. Verify the BAC water actually entered the vial.
• Cloudy or discolored solution: do not use. Discard the vial.
• Resistance or leakage at the site: withdraw, reassess site selection, and use a fresh syringe.
• Small bruise at site: normal and harmless — rotate sites next time.